Association between Glycated Hemoglobin Level and Sorting Nexin 19 Gene Polymorphism in Individuals with Type 2 Diabetes

Abstract

Background: Sorting Nexin 19 gene has an important role in different diseases and controls the transport of different hormones and peptides. But his role in type 2 diabetes is still not explored. The Sorting Nexin 19 gene is associated with insulin signaling. Therefore, the current study was conducted to investigate the association between the Sorting Nexin 19 gene and HbA1c levels in individuals with type 2 diabetes

Methods: This prospective cohort study was conducted at the Institute of Pharmaceutical Sciences, Khyber Medical University, and endocrinology department of Hayatabad Medical Complex, Peshawar. A total of 60 type 2 diabetes individuals were enrolled, and blood samples were collected from each participant. Non-probability purposive sampling was used. Blood was collected at baseline to measure HbA1c levels, DNA extraction, and conduct Sanger sequencing. After three months of follow-up, blood sample was collected to determine HbA1c level.

Results: The mean age of patients was 54.37±11.61±SD. There was no Sorting Nexin 19 rs431825173 gene mutation among the selected patients. Notably, other Sorting Nexin 19 polymorphisms on a flanking region of rs431825173, specifically rs3751036 (C>G), were detected. Out of the total 60 samples, 2 were of the CC wild type, 41 were GG homozygous, and 17 were heterozygous GC. It has been observed that rs431825173 mutation is not deleterious and has no clinical significance in type 2 diabetes.

Conclusion: The study concluded that no association was found between sorting nexin 19 gene polymorphisms and HbA1c levels and clinical outcome in type 2 diabetes patients.